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Titlebook: Viruses as Therapeutics; Methods and Protocol Alexandra R. Lucas Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 20

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A Mouse Model of Acute Liver Injury by Warm, Partial Ischemia-Reperfusion for Testing the Efficacy s known to be involved in IRI. The use of virus-derived immune-modulating proteins thus represents a promising source for new treatments to target ischemia-reperfusion injury. Laboratory small animal models of IRI are well established and are able to reproduce many aspects of ischemia-reperfusion in
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Viruses for Landscape-Scale Therapy: Biological Control of Rabbits in Australia,. therapeutics to control European rabbits (.), the preeminent invasive vertebrate pest species. Rabbits have caused major environmental and agricultural losses and contributed to extinction of native species. It was not until the introduction of Myxoma virus that effective control of this pest was
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Development and Expression of Subunit Vaccines Against Viruses in Plants, Plant-based transient protein expression systems offer an advantageous alternative to costly mammalian cell culture-based systems and can perform posttranslational modifications due to the presence of an endomembrane system that is largely similar to that of the animal cell. Technological advances
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Development of Antiviral Vaccine Utilizing Self-Destructing , for Antigen and DNA Vaccine Delivery, ability to induce long-term protective immunity against a specific virus. Bacterial vaccine vectors have been used to impart protection against self, as well as heterologous antigens. One significant benefit of using live bacterial vaccine vectors is their ability to invade and colonize deep effect
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Molecular Design and Production of AAV Viral Vectors for Gene Therapy,rapy field due to its safety profile and low immunogenicity. With a carrying capacity of 4.2 kbp, these vectors have demonstrated their clinical value, especially in the field of ophthalmology. Herein we describe methods for the molecular design and packaging of AAV viral vectors. These methods appl
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Microscopic Analysis of Viral Infection, Here we describe a set of methods to track infection spreading from one cell population to another as well as to visualize transfer of virions between cells. This approach is extended to multiplexing with physiological readouts of cell death, which can be correlated with single-cell resolution to v
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