Titlebook: Virus Hybrids as Nanomaterials; Methods and Protocol Baochuan Lin,Banahalli Ratna Book 2014 Springer Science+Business Media, New York 2014

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Tobacco Mosaic Virus Capsid Protein as Targets for the Self-Assembly of Gold Nanoparticles biological templates, such as proteins, takes advantage of the monodispersity and precision of naturally evolved systems to produce highly organized assemblies of small molecules and nanoparticles. Here we describe a method whereby arginine residues on a viral coat protein (Tobacco Mosaic Virus) ar

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Genetic Engineering and Characterization of Cowpea Mosaic Virus Empty Virus-Like Particlesed promise in CPMV capsid technologies. The recombinant nature of CPMV eVLP production means that the extent and variety of genetic modifications that may be incorporated into the particles is theoretically much greater than those that can be made to infectious CPMV virions due to restrictions on vi

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Engineered Bacteriophage T4 Nanoparticles for Cellular Imagingemical modification for tailoring the desired functionality. Dye-conjugated T4 NPs exhibiting bright fluorescence are biocompatible and can be internalized by various eukaryotic cells which land themselves as excellent cellular imaging agents. Here, we describe the preparation of engineered T4 NPs i

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Phage-Displayed Single Domain Antibodies as Recognition Elementsisplay antibody reagents. The phage-display system is routinely used to enrich for recombinant antibodies against a specific target antigen from highly diverse naïve and immune libraries. Often once binders are selected, they are expressed as soluble proteins; however, it can be advantageous to use

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Molecular Targeted Viral Nanoparticles as Tools for Imaging Cancerics, vaccines, and imaging tools. The plant virus, . (CPMV), has been successfully engineered to create novel cancer-targeted imaging agents by incorporating fluorescent dyes, polyethylene glycol (PEG) polymers, and targeting moieties. Using straightforward conjugation strategies, VNPs with high sel

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Book 2014and nonmedical applications. These include methods and techniques for genetically engineering viruses for therapeutic purpose and vaccine production, chemically modified viruses for virus-templated nanoparticles production, and genetically engineered or chemically modified viral particles as imaging