Titlebook: Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies; Mónica V. Cunha,João Inácio Book 2015 Springer Science

ALB

A Real-Time PCR Assay for the Diagnosis of Gastrointestinal Nematode Infections of Small Ruminantsderpins their control. Traditional methods of diagnosis are inaccurate, time-consuming and laborious. Here, we describe a step-by-step protocol for the molecular-based diagnosis of infections by real-time PCR.

CLASP

Improved Detection of , in Bovine Tissues Using Immunomagnetic Separation Approachesuberculosis (bTB), from tissue samples. It is a physical cell separation technique that does not impact cell viability, unlike traditional chemical decontamination prior to culture. IMS is performed with paramagnetic beads coated with .-specific antibody and peptide binders. Once captured by IMS, .

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Detection of Fish Pathogens by Loop-Mediated Isothermal Amplification (LAMP) Techniqueize the economic impact of diseases on the fish farm owners. The currently used molecular diagnostic tools of fish infectious agents, such as PCR and RT-PCR, are sensitive and specific but still have some drawbacks. These tools are usually time consuming and laborious, need skilled persons, and requ

一美元

Direct Detection of , in Bovine Blood Samples Using Standard and Isothermal DNA Amplification Approation of . pathogens in infected animals requires the microscopic examination of stained-blood smears and serological methods. Molecular diagnostic assays have been developed for the detection of . parasites, including PCR-based and reverse line blotting approaches, but these methods usually demand q

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