Titlebook: Vertebrate Embryogenesis; Embryological, Cellu Francisco J. Pelegri Book 2019Latest edition Springer Science+Business Media, LLC, part of S

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Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos,ocols for analysis of protein-DNA and protein-protein interactions in tissues and cells isolated from the developing chick. These assays are aimed at the identification of interactions between transcription factors and regulatory elements in the genome, and, in combination with functional assays, ca

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RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq),are therefore crucial for identifying novel regulators and the understanding of their function. However, in situ methods do not resolve global gene expression, and sequencing-based methods usually do not provide spatial information. Here, we describe tomo-seq, a method that combines classical histol

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Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos,to reversibly bind together an “anchor” protein and a “bait” protein. Proteins can therefore be directed to specific subcellular compartments, altering biological processes such as cell polarity and signaling. I detail methods for achieving transient expression of fusion proteins encoding the phytoc

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Ex Utero Culture and Imaging of Mouse Embryos,vailability and improvement of a wide variety of genetically encoded fluorescent proteins have provided indispensable tools to visualize cells and subcellular features in living organisms. It is now possible to generate genetically modified mouse lines expressing several spectrally distinct fluoresc

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Manipulation of Developmental Function in Turtles with Notes on Alligators,n comparative and evolutionary developmental biology makes protocols for the study of reptile embryos invaluable resources. The relatively large size, seasonal breeding, and long gestation times of turtles epitomize the challenges faced by the developmental biologist. We describe protocols for the p

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Visualizing the Balbiani Body in Zebrafish Oocytes,s isolation, histology, staining, and microscopic examination of early zebrafish oocytes. These techniques can be applied to visualize gene products that are localized to the Balbiani body, and when applied to mutants can be used to decipher molecular and genetic pathways acting in Balbiani body dev