Titlebook: Vertebrate Embryogenesis; Embryological, Cellu Francisco J. Pelegri Book 2019Latest edition Springer Science+Business Media, LLC, part of S

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1064-3745 ebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition. serves as an invaluable aid to scientists, educators, and the advanced general audience a978-1-4939-9009-2Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Culture and Host Transfer of , Oocytes for Maternal mRNA Depletion and Genome Editing Experiments,obtaining, culturing, transferring, and fertilizing . oocytes are described. This method has typically been used to alter maternal gene function by antisense oligonucleotide-mediated mRNA knockdown, but is also useful for mRNA or protein overexpression, including the expression of genome-editing rea

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Book 2019Latest editionoducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and fully updated, .Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition. serves as an invaluable aid to scientists, educators, and the advanced general audience a

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Culture and Host Transfer of , Oocytes for Maternal mRNA Depletion and Genome Editing Experiments,immediately after fertilization, it is often desirable to perturb gene function before this occurs. Oocytes can be experimentally manipulated in vitro, prior to maturation, and subsequently fertilized or otherwise activated to develop, and then observed for any embryological defects. Available metho

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Assaying NanoLuc Luciferase Activity from mRNA-Injected , Embryos, analysis of post-transcriptional regulatory events has relied upon the use of chimeric reporter mRNAs that encode firefly luciferase fused to potential regulatory sequences. A new and more sensitive luciferase developed recently called NanoLuc has the potential to improve reporter studies and provi

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Membrane Microdomains as Platform to Study Membrane-Associated Events During Oogenesis, Meiotic Matamete interaction and possibly fusion in African clawed frog .. Here we describe our protocol for identifying and analyzing molecular and cellular machinery that contributes to a variety of biological processes in the course of oogenesis, oocyte maturation, egg fertilization, and early embryogenesis

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