Titlebook: VEGF Signaling; Methods and Protocol Lorna Fiedler Book 2015 Springer Science+Business Media New York 2015 Cardiovascular Development.Lymph

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Analysis of VEGF-Mediated ERK5 Activity in Endothelial Cellspathway. It is ubiquitously expressed in mammalian cells and is activated by a number of growth factors. Gene knockout studies in mice have shown a critical role for ERK5 cardiovascular development and vascular integrity. Current methods to detect ERK5 activation in cells have relied on in vitro kin

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Vasculogenesis and Angiogenesis in VEGF Receptor-1 Deficient Micetor (PlGF). VEGFR-1 is an enigmatic molecule whose precise role in postnatal angiogenesis remains controversial. Although many postnatal and adult studies have been performed by manipulating VEGFR-1 ligands, including competitive binding by truncated VEGFR-1 protein, neutralization by antibodies, or

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The Embryonic Mouse Hindbrain and Postnatal Retina as In Vivo Models to Study Angiogenesisnd wound healing, but is also associated with different diseases such as cancer and neovascular eye disease. Accordingly, elucidating the molecular and cellular mechanisms of angiogenesis has the potential to identify new therapeutic targets to stimulate new vessel formation in ischemic tissues or i

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Generation of Targeted Mutations in Zebrafish Using the CRISPR/Cas Systemegy in zebrafish using a clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system. By injecting two simple in vitro-synthesized components [Cas9 mRNA and single guide (sgRNA)] into one-cell-stage embryos, mutations of the target gene could be efficient

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Book 2015 present a range of both firmly established and newly emerging technologies, including those that are amenable to aiding in drug discovery or pre-clinical investigations. Each of the three sections begins with an introductory overview followed by supporting relevant methods. A major advantage of thi