Titlebook: VEGF Signaling; Methods and Protocol Lorna Fiedler Book 2015 Springer Science+Business Media New York 2015 Cardiovascular Development.Lymph

EXCEL

Identification of Receptor Tyrosine Kinase Inhibitors Using Cell Surface Biotinylation and Affinity relevant readout of inhibitor activity. In this example, we use the VEGF-A–VEGFR-2 axis and the well-characterized tyrosine kinase inhibitor sunitinib as a working model; however this technique is highly applicable for the identification of inhibitors to other receptor tyrosine kinases.

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1064-3745 ation advice from the experts.This volume provides a collection of protocols for studying and manipulating VEGF signaling pathways in vitro and in vivo, and in particular, aims to present a range of both firmly established and newly emerging technologies, including those that are amenable to aiding

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Chromatin Immunoprecipitation Assay: Examining the Interaction of NFkB with the VEGF Promotern cofactors, DNA replication factors, and DNA repair proteins. Here we describe a useful ChIP-qPCR protocol to examine the interaction of NFkB with the VEGF promoter in adult rat primary cardiomyocytes that have been mechanically stretched after attaching to the extracellular matrix protein laminin.

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