Titlebook: VEGF Signaling; Methods and Protocol Lorna Fiedler Book 2015 Springer Science+Business Media New York 2015 Cardiovascular Development.Lymph

gusher

Generation of Targeted Mutations in Zebrafish Using the CRISPR/Cas Systemrategy to facilitate the synthesis of sgRNA. The system allows biallelic inactivation of multiple genes simultaneously by co-injecting a mix of sgRNAs with a single Cas9 construct. This flexible strategy of gene inactivation provides an efficient way to interrogate gene functions and genetic interactions in zebrafish.

安慰

Quantitation of Circulating Neuropilin-1 in Human, Monkey, Mouse, and Rat Sera by ELISAl-length NRP1 ECD has also been identified in human and animal sera. Here, we describe primate and rodent NRP1 ELISAs that measure total circulating NRP1 including soluble NPR1 and NRP1 ECD in human, monkey, mouse, and rat sera.

证实

泛滥

VEGF Splicing and the Role of VEGF Splice Variants: From Physiological-Pathological Conditions to Spn since its expression is controlled at all possible steps including transcription, mRNA stability, translation, and pre-mRNA splicing. The latter form of molecular regulation is probably the least studied. This field has been neglected; yet different forms of VEGF with different sizes and different

BLAZE

Detection and Quantification of VEGF Isoforms by ELISAs. The human VEGF gene has eight exons. Different VEGF isoforms are expressed via alternative RNA splicing and VEGF. and VEGF. are the major isoforms present in human tissues. The exact roles of these different VEGF isoforms are not totally clear. Assays to detect specific VEGF isoforms in biologica

Gentry

抗原

Intractable

Induction of VEGF Secretion in Cardiomyocytes by Mechanical Stretchjecting cells to in vitro mechanical stretch can mimic cellular responses to changes in the rigidity of the extracellular matrix. Here we describe an in vitro model system that mimics stretch overload in vivo. In this stretch-mediated hypertrophy model, adult rat cardiomyocytes attached to laminin-c

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