Titlebook: VEGF Signaling; Methods and Protocol Lorna R. Fiedler,Caroline Pellet-Many Book 2022Latest edition The Editor(s) (if applicable) and The Au

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Multiparameter Fluorescence-Activated Cell Sorting of Human Lymphatic Endothelial Cells,D34.CD31.VEGFR-3.Podoplanin. profile that permits their separation from blood vascular endothelial cells and other cells likely to be present in the digested tissue sample. In addition, FACS allows the evaluation of LEC size, volume, granularity, and purity at the time of sorting.

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VEGF-A165 -Induced Endothelial Cells Chemotactic Migration and Invasion Assays,arated with a porous membrane through which cells can migrate. The lower compartment contains the chemoattractant, creating a gradient by diffusing into the upper chamber containing the cells. Adherent cells will migrate through the membrane and remain on the lower side of the membrane, where they can finally be fixed, stained, and counted.