Titlebook: VEGF Signaling; Methods and Protocol Lorna R. Fiedler,Caroline Pellet-Many Book 2022Latest edition The Editor(s) (if applicable) and The Au

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Global MicroRNA Profiling of Vascular Endothelial Cells,cies. In roughly half of the cases, the most abundant isomiR in the cells is not the reference miRNA given in miRBase, which highlights the importance of isomiR-specific analysis. Here, we describe a gel-free protocol for global miRNA profiling in vascular endothelial cells and the main steps of the

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VEGF-A165 -Induced Endothelial Cells Chemotactic Migration and Invasion Assays,en chamber assay was developed to investigate chemotaxis, which corresponds to the directional migration of cells in response to a chemoattractant gradient. It is a reliable and convenient assay that does not require expensive equipment..In the modified Boyden chamber assay, two compartments are sep

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Measuring Mitochondrial Calcium Fluxes in Cardiomyocytes upon Mechanical Stretch-Induced Hypertrophences signaling cascades involved in cell metabolism and cell survival. Prolonged dysregulation of mitochondrial Ca2+ leads to dysfunctional cardiomyocytes, apoptosis and ultimately heart failure. VEGF promotes cardiomyocyte contractility by increasing calcium transients to control the strength of t

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PET-scan

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