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Titlebook: Urinary Enzymes; in Clinical and Expe Klaus Jung,Hermann Mattenheimer,Ulf Burchardt Book 1992 Springer-Verlag Berlin Heidelberg 1992 Enzymd

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Origin of Enzymes in Urineood plasma, blood cells, bacteria, epithelium of the urinary tract, and seminal fluid. A classification of diagnostically relevant urinary enzymes according to their origin and handling by the kidney is given in Table 1.
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Distribution of Diagnostically Relevant Enzymes Along the Nephronnter the urinary compartment by glomerular filtration, tubular or postrenal release. From the study of renal morphology, biochemistry and function, our knowledge concerning the intranephron and intracellular origin of enzymes has greatly increased over the past decades [15, 31]. Because of their rel
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Mechanisms of Enzyme Release and Causes of Altered Enzyme Excretion basic principles can be deduced for changes of enzymuria: (a) abnormal composition of the primary urine, independent of the cause for any alterations, which may be pre-renal (e.g., increased level of lysozyme) or renal (e.g., increased glomerular permeability), and (b) structural and functional abe
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Preanalytical Treatment of Urine enzymes, which are protected by a relatively stable “enzyme friendly” milieu in a closed system, enzymes from kidney cells lining the tubular lumen are released into an open system and encounter in the urine an “enzyme hostile” environment, which may alter the molecular structure of enzyme proteins
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Methods for Measuring Urinary Enzyme Activitiesptide chains. Two, namely the cytosolic (EC 3.4.11.1) and the microsomal forms (EC 3.4.11.2), are of relatively broad substrate specificity. The cytosolic enzyme, the “classic” leucine aminopeptidase [6], hydrolyzes .-peptides with an N-terminal leucine residue. The recommended substrates are .-leuc
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Reference Intervalshap. 8). They should be regarded as guidelines for the clinician and clinical chemist. It must be considered that these values may vary significantly due to methodological differences as especially observed in all cases of enzyme activity measurements. Thus, the methods are briefly characterized by
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Urinary Enzymes in Renal, Renal-Associated and Urological Disordersand/or structural integrity of the kidney. Clinicians demand cost-efficient tests which sensitively and specifically reflect renal function and do not annoy patients. Highly specialized techniques like biopsy, arteriography and pyelography, although revealing specific and often unique information, r
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