书目名称 | The Nucleus | 副标题 | Volume 1: Nuclei and | 编辑 | Ronald Hancock | 视频video | | 概述 | Includes state-of-the-art procedures for isolating and working with nuclei from a wide range of cell types, and.procedures for studying their components including pathology-related structures.Includes | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned. The application and continuing development of the new, powerful biochemical and biophysical methodologies described here are essential in this quest. In The Nucleus, researchers from more than forty leading international laboratories describe state-of-the-art methods for isolating nuclei and their components and for studying their structure and activities, including some pathology-associated features. Volume I: Nuclei and Subnuclear Structures presents an overview of features of the intranuclear environment, followed by the most recent procedures for isolating nuclei from a wide range of cell types including muscle cells, yeast, oocytes, cells with polytene nuclei, Arabidopsis, trypanosomes, and dinoflagellates. The latest methods are described for isolating and working with nucleoli, constitutive heterochromatin, pathology-associated inclusions, and chromatin and for examining glycosylation, sumoylation, and ADP-ribosylation of nuclear proteins. Written in the highly successful Methods in Molecular Biology™ ser | 出版日期 | Book 2008 | 关键词 | DNA; FISH; Interphase chromosomes; Intranuclear structures; Isolation; Nucleolus; RNA; Telomere; cell nucleu | 版次 | 1 | doi | https://doi.org/10.1007/978-1-59745-406-3 | isbn_softcover | 978-1-61737-863-8 | isbn_ebook | 978-1-59745-406-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Humana Press 2008 |
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