| 书目名称 | The CRAC Channel | | 副标题 | Methods and Protocol | | 编辑 | Aubin Penna,Bruno Constantin | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | This volume discusses the cellular and molecular techniques used to study and characterize the different components of the CRAC channel signaling pathway. The chapters in this book cover topics such as fluorescence-based measurements of the CRAC channel activity in cell populations; patch-clamp recording of the CRAC channel in STIM-Orai overexpressing cells and native systems; western-blotting and co-immunoprecipitation of endogenous STIM/ORAI and protein partners; shRNA-mediated gene silencing; and the SC-SMD system. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Thorough and cutting-edge, .The CRAC Channel: Methods and Protocols. is a valuable resource for any researcher interested in learningmore about the CRAC channel, as its activation mechanism and roles in cellular functions have yet to be completely explored.. | | 出版日期 | Book 2018 | | 关键词 | cytosolic; endoplasmic reticulum Ca2+; Ca2+ influx; Ca2+ release-activated Ca2+ current; STIM stimulatin | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-8704-7 | | isbn_softcover | 978-1-4939-9370-3 | | isbn_ebook | 978-1-4939-8704-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2018 |
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发表于 2025-3-21 18:35:37
