Titlebook: Systemic Lupus Erythematosus; Methods and Protocol Paul Eggleton,Frank J. Ward Book 2014 Springer Science+Business Media New York 2014 Mole

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MAG

Bifunctional Antibody Fragment-Based Fusion Proteins for the Targeted Elimination of Pathogenic T-Cg, e.g., methotrexate, prednisolone, or TNF blockers, has prominent therapeutic effects frequently at the cost of severe long-term side effects and toxicity. Therefore, targeted strategies that can selectively inhibit or eliminate pathogenic T cells are sought after as a new approach to safely block

Herpetologist

,TLC Immunostaining for Detection of “Antiphospholipid” Antibodies,spholipid syndrome (APS) is the most frequently acquired thrombophilia, characterized by thrombosis and obstetric manifestations associated to an autoimmune trait, represented by the positivity of antiphospholipid (aPL) antibodies. Immunoassays for anticardiolipin (aCL) and anti-β2 glycoprotein I (a

MAUVE

Induced Murine Models of Systemic Lupus Erythematosus,s used in the pristane-induced model and the chronic graft-versus-host disease (cGVHD) model, both of which have been extensively used. We will also outline the specific mechanisms of systemic autoimmunity that can be best characterized using each of these models.

Hyperplasia

Measuring Interferon Alpha and Other Cytokines in SLE, I interferon (IFN), specifically IFN-α, is recognized as a central mediator of disease pathogenesis in SLE. We describe a functional assay to measure type I IFN activity in SLE plasma and have also measured the response of peripheral blood cells to that cytokine family. This method can be scaled to

谎言

Detection of SLE Antigens in Neutrophil Extracellular Traps (NETs),decorated with granular proteins called neutrophil extracellular traps (NETs) has recently been implicated in the pathogenesis of inflammatory and autoimmune diseases. Indeed, NETs may represent an important source of autoantigens and immunostimulatory proteins in systemic lupus erythematosus (SLE).

canonical

Detection and Characterization of Autoantibodies Against Modified Self-Proteins in SLE Sera After Eosttranslationally modified forms. In recent years, these autoantibodies have provoked interest as initiators of pathology and as biomarkers of disease activity. Often, the host antigens employed in lab-based and commercially developed immunoassays use non-human antigen or non-modified host antigen

inveigh

Generation of Self-Peptides to Treat Systemic Lupus Erythematosus,to enter in clinical trials for chronic autoimmune diseases and systemic lupus erythematosus (SLE) in particular. Here, we describe and discuss a series of assays, which may help in characterizing valuable candidate peptides that were applied in our laboratory to develop the lupus P140 peptide progr

重力

啤酒

Evaluating a Particular Circulating MicroRNA Species from an SLE Patient Using Stem-Loop qRT-PCR, treatment. Circulating microRNAs (miRNAs) could serve as potential biomarkers of disease activity or status in SLE, and here we describe a modified qRT-PCR method for detecting them. Stem loop has become one of the most powerful methods for determining miRNA expression because it is highly sensitiv