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Titlebook: Synthetic mRNA; Production, Introduc Robert E. Rhoads Book 2016 Springer Science+Business Media, LLC, part of Springer Nature 2016 gene the

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发表于 2025-3-21 17:33:51 | 显示全部楼层 |阅读模式
书目名称Synthetic mRNA
副标题Production, Introduc
编辑Robert E. Rhoads
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Synthetic mRNA; Production, Introduc Robert E. Rhoads Book 2016 Springer Science+Business Media, LLC, part of Springer Nature 2016 gene the
描述.This volumepresents detailed laboratory protocols for .invitro. synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy. Synthetictechniques are described for structural features in mRNA that provide investigationaltools such as fluorescence emission, click chemistry, photo-chemicalcrosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reducedactivation of the innate immune response. Protocols are described for clinicalapplications such as large-scale transfection of dendritic cells, production ofGMP-grade mRNA, redirecting T cell specificity, and use of molecularadjuvants for RNA vaccines. Writtenin the highly successful .Methods inMolecular Biology. series format, chapters include introductions to theirrespective topics, lists of the necessarymaterials and reagents, step-by-steplaboratory protocols, and tips on troubleshooting and avoiding known pitfalls..SyntheticmRNA: Production, Introduction into Cells, and Physiological Consequences .is a valuable and cutting-e
出版日期Book 2016
关键词gene therapy; DNA; lineage conversion of cell fates; genome editing; mRNA; protein replacement therapy; Im
版次1
doihttps://doi.org/10.1007/978-1-4939-3625-0
isbn_softcover978-1-4939-8098-7
isbn_ebook978-1-4939-3625-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2016
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f 2008. These crises have been metaphorically interpreted as “black swans” by Nassim Taleb in a book that made a worldwide impression, . (.). A “black swan” is an event that is very unlikely and not normally to be expected (i.e., an “outlier”) but which, if it does occur, will have extreme and possi
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ncluding the reversal of the oil companies’ attitude in the early 1990s and the current fossil fuel industry’s standpoint. It goes on to show that the global transition to carbon neutrality is possible, desirable and cost-effective in the long term but that the mobilization of society, companies and
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Joanna Kowalska,Franck Martin,Jacek Jemielityncluding the reversal of the oil companies’ attitude in the early 1990s and the current fossil fuel industry’s standpoint. It goes on to show that the global transition to carbon neutrality is possible, desirable and cost-effective in the long term but that the mobilization of society, companies and
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Josephin M. Holstein,Daniela Stummer,Andrea Rentmeistererge of what many are calling the "second information revolution," based on ubiquitous access to both computing and information. Handheld communication devices will become portable and even wearable remote control devices for both the social and physical worlds. At the same time, access to informati
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Daphné Benteyn Ph.D.,Carlo Heirman,Kris Thielemans,Aude Bonehillerge of what many are calling the "second information revolution," based on ubiquitous access to both computing and information. Handheld communication devices will become portable and even wearable remote control devices for both the social and physical worlds. At the same time, access to informati
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