书目名称 | Synthetic mRNA | 副标题 | Production, Introduc | 编辑 | Robert E. Rhoads | 视频video | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .This volumepresents detailed laboratory protocols for .invitro. synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy. Synthetictechniques are described for structural features in mRNA that provide investigationaltools such as fluorescence emission, click chemistry, photo-chemicalcrosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reducedactivation of the innate immune response. Protocols are described for clinicalapplications such as large-scale transfection of dendritic cells, production ofGMP-grade mRNA, redirecting T cell specificity, and use of molecularadjuvants for RNA vaccines. Writtenin the highly successful .Methods inMolecular Biology. series format, chapters include introductions to theirrespective topics, lists of the necessarymaterials and reagents, step-by-steplaboratory protocols, and tips on troubleshooting and avoiding known pitfalls..SyntheticmRNA: Production, Introduction into Cells, and Physiological Consequences .is a valuable and cutting-e | 出版日期 | Book 2016 | 关键词 | gene therapy; DNA; lineage conversion of cell fates; genome editing; mRNA; protein replacement therapy; Im | 版次 | 1 | doi | https://doi.org/10.1007/978-1-4939-3625-0 | isbn_softcover | 978-1-4939-8098-7 | isbn_ebook | 978-1-4939-3625-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2016 |
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