Titlebook: Synthetic Antibodies; Methods and Protocol Thomas Tiller Book 2017 Springer Science+Business Media LLC 2017 Amino acid based antibodies.Aff

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Selection of Aptamers Against Whole Living Cells: From Cell-SELEX to Identification of Biomarkers assays. Furthermore, we also provide a protocol to purify and identify the target of these aptamers. These protocols could be useful for the discovery of lead therapeutic compounds and diagnostic cell-surface biomarkers.

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Book 2017g, aptamer development, and generation of molecularly imprinted polymers (MIPs). Part I includes methods that deal with amino-acid based synthetic antibodies. Brief protocols about the generation of antibody libraries are detailed, as well as techniques for antibody selection, characterization, and

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Generating Conformation and Complex-Specific Synthetic Antibodiesntibodies for future therapeutic use. The methods described are also applicable to screening directly on cells and can be ported to other in vitro directed evolution systems utilizing non-immunoglobulin scaffolds.

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Whole Cell Panning with Phage Displaye panning), whole cell panning is an elegant way to present membrane embedded target antigens in their natural environment and conformation to antibody-bearing phages. Here, a whole cell panning procedure using a Fab-based antibody library including primary cell based screening for selectivity is described.

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A Biotin Ligase-Based Assay for the Quantification of the Cytosolic Delivery of Therapeutic Proteinsstuck in the endosomes and those that have escaped into the cytosol. Here, we describe a method employing the prokaryotic enzyme biotin ligase that overcomes this problem and which can be employed for a highly sensitive quantification of cytosolic protein delivery.