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Titlebook: Synthetic Antibodies; Methods and Protocol Thomas Tiller Book 2017 Springer Science+Business Media LLC 2017 Amino acid based antibodies.Aff

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发表于 2025-3-21 17:05:08 | 显示全部楼层 |阅读模式
书目名称Synthetic Antibodies
副标题Methods and Protocol
编辑Thomas Tiller
视频video
概述Includes cutting-edge methods and protocols for the study of synthetic antibodies.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the
丛书名称Methods in Molecular Biology
图书封面Titlebook: Synthetic Antibodies; Methods and Protocol Thomas Tiller Book 2017 Springer Science+Business Media LLC 2017 Amino acid based antibodies.Aff
描述This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs). Part I includes methods that deal with amino-acid based synthetic antibodies. Brief protocols about the generation of antibody libraries are detailed, as well as techniques for antibody selection, characterization, and validation. This section is completed by a brief description of a bioinformatics platform that supports antibody engineering during research and development. Part II contains basic procedures about the selection and characterization of aptamer molecules, and Part III describes fundamental processes of MIP generation and application. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Synthetic Antibodies: Methods and Protocols. is an ideal guide for scientists seeking to pr
出版日期Book 2017
关键词Amino acid based antibodies; Affinity reagents; Aptamers; Nucleotides; Moleculary imprinted polymers; Imm
版次1
doihttps://doi.org/10.1007/978-1-4939-6857-2
isbn_softcover978-1-4939-8319-3
isbn_ebook978-1-4939-6857-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media LLC 2017
The information of publication is updating

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Novel HPLC-Based Screening Method to Assess Developability of Antibody-Like Moleculeses that, in addition to having excellent biological properties, also have optimal biophysical characteristics. Here, we describe a simple HPLC-based screening method to assess for developability factors earlier in the discovery process.
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978-1-4939-8319-3Springer Science+Business Media LLC 2017
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Construction of a scFv Library with Synthetic, Non-combinatorial CDR Diversityn-combinatorially synthesized CDR sequences. Each CDR is first inserted to a master scFv framework sequence and the resulting single-CDR libraries are subjected to a round of proofread panning. The proofread CDR sequences are assembled to produce the final scFv library with six diversified CDRs.
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Enzymatic Assembly for scFv Library Constructionmbinant antibodies through biopanning. Here, we describe a simple and efficient method for assembling V. and V. fragments through the concerted action of λ-exonuclease and . DNA polymerase. We successfully used this method to construct a diverse chicken scFv library.
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