Titlebook: Surface Plasmon Resonance; Methods and Protocol Nico J. Mol,Marcel J. E. Fischer Book 2010 Humana Press 2010 Binding modes.Biomolecular int

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Surface Plasmon Resonance Spectroscopy in Determination of the Interactions Between Amyloid , Proteecular pathogenesis of amyloidoses have shown that the plasma membrane plays an important role in amyloidogenesis and cytotoxicity induced by amyloidogenic proteins. By immobilizing lipid bilayers on a sensor chip surface, SPR spectroscopy has been employed to examine the binding of amyloidogenic pr

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Incorporation of a Transmembrane Protein into a Supported 3D-Matrix of Liposomes for SPR Studies,sensitivity compared to traditional biomedical methods. For the use of SPR as a biomedical research tool there is a need to immobilize the reactants to a solid sensor surface. It is nowadays fairly straightforward to immobilize various reactants and hydrophilic proteins to a solid sensor surface and

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SPR/MS: Recovery from Sensorchips for Protein Identification by MALDI-TOF Mass Spectrometry, active concentrations. However, the technology cannot identify the involved interaction partners. Mass spectrometry, on the other hand, can be used for specific identification of proteins in amounts comparable to the levels that can be captured on a Biacore SPR sensorchip. Here we present protocols

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A Capture Coupling Method for the Covalent Immobilization of Hexahistidine Tagged Proteins for Surfmmobilization using high-affinity interactions such as biotin/streptavidin or antibody/antigen. In this chapter we describe a robust method to covalently immobilize His. fusion proteins on the sensor surface for SPR analysis.

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SPR/MS: Recovery from Sensorchips for Protein Identification by MALDI-TOF Mass Spectrometry,or specific identification of proteins in amounts comparable to the levels that can be captured on a Biacore SPR sensorchip. Here we present protocols for capturing, washing and eluting proteins from Biacore instruments as well as for robust sample preparation for sensitive mass spectrometric identification.

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