Titlebook: Surface Plasmon Resonance; Methods and Protocol Nico J. Mol,Marcel J. E. Fischer Book 2010 Humana Press 2010 Binding modes.Biomolecular int

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A Capture Coupling Method for the Covalent Immobilization of Hexahistidine Tagged Proteins for Surfomponent must be immobilized on a sensor surface. This is typically achieved using covalent immobilization via free amines or thiols, or noncovalent immobilization using high-affinity interactions such as biotin/streptavidin or antibody/antigen. In this chapter we describe a robust method to covalen

Alveoli

Affinity Constants for Small Molecules from SPR Competition Experiments,attractive alternative. A small ligand known to bind to the analyte is immobilized on an SPR sensor surface, and the binding of the larger analyte in the presence of compounds under investigation in a concentration range is assayed. The resulting inhibition curves of the equilibrium SPR signal as fu

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SPR Biosensor as a Tool for Screening Prion Protein Binders as Potential Antiprion Leads, No effective treatments are currently available for the diseases, vCJD in particular. It is believed that the formation of protease-resistant insoluble prion protein (PrP.), which is the main component of amyloidal deposits, from the cellular prion protein (PrP.), is essential for the progression o

AMBI

,Carbohydrate–Lectin Interactions Assayed by SPR,tins. The main interest of using such an approach is that neither the lectins – which are proteins – nor their ligands – natural compounds such as glycoproteins, oligosaccharides, polysaccharides, or synthetic glycoconjugates such as glycoclusters or neoglycoproteins – require any tag. Because lecti

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DNA Sensors Based on Mixed Self-Assembled DNA/Alkanethiol Films,egies (e.g., adsorption, complexation, and entrapment) can only be used on standard microscope slides, while for more tailored surfaces alternative strategies are still required. In the case of gold surfaces, the self-assembly of mixed DNA/alkanethiol films is a very common approach to directly coup

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Preparation of Lipid Membrane Surfaces for Molecular Interaction Studies by Surface Plasmon Resonanto study protein–protein interactions, but in recent years membrane model systems have also become available and this makes it possible to study protein–membrane interactions as well. In this review chapter we describe possible ways to prepare lipid membrane surfaces on various sensor chips and some

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,Capture of Intact Liposomes on Biacore Sensor Chips for Protein–Membrane Interaction Studies,working with lipids and membranes: the L1 and HPA sensor chips. The L1 chip is the most frequently used in protein–membrane interaction studies and it allows the capture of intact liposomes. This chapter describes the protocol for immobilization of liposomes on L1 sensor chips and discusses some of

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