Titlebook: Superantigens; Methods and Protocol Amanda J. Brosnahan Book 2016 Springer Science+Business Media New York 2016 cell signaling.staphylococc

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Monkey Feeding Assay for Testing Emetic Activity of Staphylococcal Enterotoxin,diarrhea, oral administration of SEs in the monkey feeding assay is considered as a standard method to evaluate emetic activity of SEs. This chapter summarizes and discusses practical considerations of the monkey feeding assay used in studies characterizing classical and newly identified SEs.

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,Quantification of a Selective Expansion of T Cell Receptor Vβ by Superantigen Using Real-Time PCR,-time PCR method using SYBR green I and primers specific to Cβ and Vβ genes for an absolute quantification. The established method was applied to quantify a selective expansion of T cell receptor Vβ expansion by superantigens and generated accurate, reproducible, and comparable results.

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Book 2016cular biology, biochemistry, and cellular biology, with a strong focus on disease models utilized to determine the role of superantigens in human disease. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists o

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Animal Models Used to Study Superantigen-Mediated Diseases,cit a massive cytokine release and activation of T cells in higher numbers than that seen with ordinary antigens. Because of this unique ability, superantigens have been implicated as etiological agents for many different types of diseases, including toxic shock syndrome, infective endocarditis, pne

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Crystallization and Structure Determination of Superantigens and Immune Receptor Complexes,on. This technique requires growing large and highly ordered crystals of the superantigen or receptor–superantigen complex, followed by exposure to X-ray radiation and data collection. Here, we describe methods for crystallizing superantigens and superantigen–receptor complexes using the vapor diffu

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Lipopolysaccharide-Induced Toxic Shock Syndrome in Rabbits, there are 24 identified staphylococcal superantigens (SAgs), some of which have yet to be fully characterized. Testing the capacity of superantigens to potentiate LPS sensitivity is essential to characterize the role of these proteins in disease development. Here we describe how to perform studies