书目名称 | Superantigen Protocols | 编辑 | Teresa Krakauer | 视频video | | 概述 | Includes supplementary material: | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | Superantigens, a class of proteins produced by bacteria and microbes, cause diseases ranging from food poisoning to toxic shock, but can also be used to understand these pathologies and devise therapeutic agents to combat them. In Superantigen Protocols, leading researchers in the biological, chemical, and physical investigation of superantigens describe in step-by-step detail their best experimental techniques to assess the physical characteristics and biological effects of these potent activators of the immune system. The protocols range from those for investigating the interactions of superantigens with cellular receptors to those for the analysis of their immunological and biological effects, including methods for using BIOcore to determine binding kinetics and establishing various lymphocyte cell culture systems. There are also accounts of such methods as the RNase protection assay, cytokine ELISA, FACS analysis, and cytokine production at the single cell level. Additional techniques for the discovery of new activators of the immune system, the development of new therapeutics for the suppression of superantigen-induced effects, and the treatment of inflammatory diseases are de | 出版日期 | Book 2003 | 版次 | 1 | doi | https://doi.org/10.1385/1592593674 | isbn_softcover | 978-1-4899-3823-7 | isbn_ebook | 978-1-59259-367-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media New York 2003 |
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