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Titlebook: Superantigen Protocols; Teresa Krakauer Book 2003 Springer Science+Business Media New York 2003

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发表于 2025-3-21 16:32:23 | 显示全部楼层 |阅读模式
书目名称Superantigen Protocols
编辑Teresa Krakauer
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Superantigen Protocols;  Teresa Krakauer Book 2003 Springer Science+Business Media New York 2003
描述Superantigens, a class of proteins produced by bacteria and microbes, cause diseases ranging from food poisoning to toxic shock, but can also be used to understand these pathologies and devise therapeutic agents to combat them. In Superantigen Protocols, leading researchers in the biological, chemical, and physical investigation of superantigens describe in step-by-step detail their best experimental techniques to assess the physical characteristics and biological effects of these potent activators of the immune system. The protocols range from those for investigating the interactions of superantigens with cellular receptors to those for the analysis of their immunological and biological effects, including methods for using BIOcore to determine binding kinetics and establishing various lymphocyte cell culture systems. There are also accounts of such methods as the RNase protection assay, cytokine ELISA, FACS analysis, and cytokine production at the single cell level. Additional techniques for the discovery of new activators of the immune system, the development of new therapeutics for the suppression of superantigen-induced effects, and the treatment of inflammatory diseases are de
出版日期Book 2003
版次1
doihttps://doi.org/10.1385/1592593674
isbn_softcover978-1-4899-3823-7
isbn_ebook978-1-59259-367-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2003
The information of publication is updating

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发表于 2025-3-21 22:09:57 | 显示全部楼层
Superantigen Protocols978-1-59259-367-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Methods in Molecular Biologyhttp://image.papertrans.cn/t/image/881758.jpg
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Teresa KrakauerIncludes supplementary material:
发表于 2025-3-22 16:58:41 | 显示全部楼层
T-Lymphocyte Activation Induced by Staphylococcal Enterotoxin Superantigens, one MHC allele. Therefore, a . any MHC class II positive cell would be suitable as superantigen presenting cell. (2) Processing of superantigens into antigenic peptides is not necessary. Therefore, just short incubations are necessary to pulse APCs with enterotoxin superantigens.
发表于 2025-3-22 19:59:47 | 显示全部楼层
Assessment of Specific T-Cell Activation by Superantigens,outline two methods for assessing specific T-cell activation based on the preceding criteria: 1) detection of markers of activation in individual TCRBV subsets early after SAg stimulation; and 2) analysis of cytokine release from TCRBV specific T-cell hybridomas in response to SAg presentation.
发表于 2025-3-22 23:41:54 | 显示全部楼层
Book 2003to understand these pathologies and devise therapeutic agents to combat them. In Superantigen Protocols, leading researchers in the biological, chemical, and physical investigation of superantigens describe in step-by-step detail their best experimental techniques to assess the physical characterist
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