Titlebook: Structural Proteomics; High-Throughput Meth Raymond J. Owens Book 2015Latest edition Springer Science+Business Media New York 2015 Bioinfor

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Screening of Stable G-Protein-Coupled Receptor Variants in ,ets. Recent successes in the determination of GPCR structures have relied on the stabilization of receptors to overcome the difficulties in expression and purification. Although a large quantity of purified protein is needed for structural determination, the majority of wild-type GPCRs are too unsta

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官僚统治

GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus Systemed approach is to fuse the protein to green fluorescent protein (GFP), enabling expression to be tracked without the need to purify the protein. Combining fusion to green fluorescent protein with the baculovirus expression system provides a useful platform for both screening and production of eukary

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Methods for the Successful Crystallization of Membrane Proteins membrane proteins by X-ray crystallography. The advent of genomics and proteomics initiatives combined with high-throughput technologies, such as automation, miniaturization, integration, and third-generation synchrotrons, has enhanced membrane protein structure determination rate. Nevertheless, cr

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Application of In Situ Diffraction in High-Throughput Structure Determination Platforms machinery of the cell. Since the emergence of structural genomics initiatives, significant advances have been made in all key steps of the structure determination process. In particular, third-generation synchrotron sources and the application of highly automated approaches to data acquisition and

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CD Spectroscopy: An Essential Tool for Quality Control of Protein Foldingotein (HTP) methods, which enable a large number of crystallization conditions to be rapidly investigated. Monitoring sample quality and the effect of crystallization buffers on protein behavior in solution should be considered as part of the crystallization experiment. Circular Dichroism (CD) spect

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Scintigraphy

Protein Structure Annotation Resourcesuctural annotation databases, as well as the range of information they provide. The different levels of annotation data (primary vs. derived vs. inferred) and how they should all be considered accordingly will also be described.

ALIAS

Characterization and Production of Protein Complexes by Co-expression in ,en be used to reconstitute protein/nucleic acid complexes in vitro. In this chapter we present protocols for the widely utilized ACEMBL and pET-MCN/pET-MCP vector series which enable the rapid and automated co-expression of protein complexes in ..

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