Titlebook: Structural Proteomics; High-Throughput Meth Raymond J. Owens Book 2015Latest edition Springer Science+Business Media New York 2015 Bioinfor

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GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus Systemotic membrane proteins. In this chapter we describe our protocol for the expression screening of membrane proteins in insect cells using fusion to GFP as a reporter. We use both SDS-PAGE with in-gel fluorescence imaging and fluorescence-detection size-exclusion chromatography (FSEC) to screen for expression.

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Book 2015Latest editiond to particularly challenging proteins, notably integral membrane proteins and multi-protein complexes. .Structural Proteomics: High-Throughput Methods, Second Edition. begins by exploring the resources available for curation, annotation, and structure prediction in silico, and continues with method

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Methods for the Successful Crystallization of Membrane Proteins high-throughput methods easily available to any researcher interested in membrane protein characterization and crystallization. It is our hope this chapter can be used as a positive guide to all who are attempting crystallizing membrane proteins.

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Cell-Free Protein Synthesis Systems Derived from Cultured Mammalian CellsK562-based cell-free system) and 50 μg/ml (CHO-based cell-free system) of active firefly luciferase are obtained in the coupled transcription-translation systems within 3 h. As a result, both cell-free protein synthesis systems serve as powerful tools for high-throughput proteomics.

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CD Spectroscopy: An Essential Tool for Quality Control of Protein Foldings of conformational folding is often disregarded in protein production. Examples of batch-to-batch variation in the local tertiary structure of aromatic side chain residues revealed by CD will be discussed. In some of the examples, the fact that ligand binding properties were affected by changes in