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Titlebook: Structural Genomics; General Applications Yu Wai Chen Book 2014 Springer Science+Business Media, LLC 2014 macromolecular machinery.protein

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发表于 2025-3-21 18:24:55 | 显示全部楼层 |阅读模式
书目名称Structural Genomics
副标题General Applications
编辑Yu Wai Chen
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Structural Genomics; General Applications Yu Wai Chen Book 2014 Springer Science+Business Media, LLC 2014 macromolecular machinery.protein
描述.The field of Structural Genomics has produced many technological advances that transform and accelerate structure solution and analysis. .Structural Genomics: General Applications. .emphasizes the benefits to the wider structural research community. It also reflects the current trend in tackling the more ambitious challenges of studying macromolecular machineries and complexes. Divided into three convenient sections, topics include the cloning and production of proteins for structural studies, experimental methods, and computational methods and data analysis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls..Authoritative and easily accessible, .Structural Genomics: General Applications. .aims primarily to channel spin-off technologies to the average structural biologist in a small or medium-sized laboratory..
出版日期Book 2014
关键词macromolecular machinery; protein production; recombinant human proteins; structural biology; structural
版次1
doihttps://doi.org/10.1007/978-1-62703-691-7
isbn_softcover978-1-4939-6060-6
isbn_ebook978-1-62703-691-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2014
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Cell-Free Expression of Protein Complexes for Structural Biology controlled. In this chapter, we describe techniques for the expression of protein complexes by an . cell-free protein synthesis system, which has been successfully utilized in crystallographic structural studies.
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Cell-Free Protein Synthesis for Functional and Structural Studiesues for determining if the target is suitable for scale-up to amounts potentially needed for structure determination. The cell-free system is versatile and can be easily customized with the inclusion of additives. We describe simple modifications used for producing membrane proteins.
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DisMeta: A Meta Server for Construct Design and Optimizationdulating intermolecular recognition processes. From a practical point of view, however, such disordered regions often can pose significant challenges for crystallization. Disordered regions are also detrimental to NMR spectral quality, complicating the analysis of resonance assignments and three-dim
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Stable Expression Clones and Auto-Induction for Protein Production in ,ed induction, inadvertently generating cultures that give poor or variable yields in protein production. Recipes are provided for (1) defined culture media in which expression strains grow to saturation without induction, thereby ensuring stable frozen stocks and seed cultures with high fractions of
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