Titlebook: Structural Genomics; General Applications Yu Wai Chen Book 2014 Springer Science+Business Media, LLC 2014 macromolecular machinery.protein

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Methods to Refine Macromolecular Structures in Cases of Severe Diffraction Anisotropyion extended to 2.1 Å resolution along a* and c* directions but only to 3.0 Å along the b* direction. Severe anisotropy such as this is often associated with lack of detail in electron density maps, stalled model improvement, and poor refinement statistics. Published methods for overcoming these dif

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A Cost-Effective Protocol for the Parallel Production of Libraries of 13CH3-Specifically Labeled Mutcombined with improved NMR spectroscopy enable nowadays studies of proteins complexes up to 1 MDa. For such large complexes, the major interest lies in obtaining structural, dynamic and interaction information in solution, which requires sequence-specific resonance assignment of NMR signals. While s

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High-Throughput Expression Screening and Purification of Recombinant Proteins in ,cale, there is the potential to use the purified proteins for characterization in pilot studies, for sensitive micro-assays, or for the quick detection of and differentiation of the expected size and oxidation state of the protein by mass spectrometry.

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Methods to Refine Macromolecular Structures in Cases of Severe Diffraction Anisotropyof anisotropy, and then applies ellipsoidal resolution boundaries, anisotropic scaling, and B-factor sharpening to the data set to compensate for the deleterious effects of diffraction anisotropy. Here, I offer advice on implementing these methods to facilitate refinement of macromolecular structures in cases of severely anisotropic data.

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Expression Screening in Mammalian Suspension Cellstions are varied to identify the optimal expression conditions. This requires multi-parallel expression screening in mammalian cells similar to that already described for .. . expression. Here we describe in detail a multi-parallel method to express proteins in mammalian suspension cells by transient transfection in 24-well blocks.

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