Titlebook: Streptococcus pneumoniae; Methods and Protocol Federico Iovino Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019

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Immunofluorescent Staining and High-Resolution Microscopy to Study the Pneumococcal Cellcent years, there have been important advances in the microscopy equipment used for imaging, and we can now perform so-called high-resolution microscopy. Through high-resolution microscopy we can not only study biological processes but also visualize them.

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High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Inteant tool that allows for visualization of biological and pathological processes occurring in vivo. As described in Chap. 5, imaging of animal and human tissue postmortem can be performed at high resolution. Recently, imaging of human tissues infected with pneumococci using an even higher resolution,

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Natural Genetic Transformation: A Direct Route to Easy Insertion of Chimeric Genes into the Pneumocond negative selection strategies have been described to create directed markerless modifications, including deletions, insertions, or point mutations. Transformation with DNA fragments carrying long flanking homology sequences is also used to generate mutations without selection but it requires high

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Mass Spectrometry to Study the Bacterial Proteome from a Single Colonyltures of bacteria grown from single cells. In this chapter, we describe a method for identifying bacteria at the species level with 100% accuracy using the proteomes of bacterial cultures from single colonies. In this chapter, six reference strains of gram-negative and gram-positive bacteria are an

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In Vitro Adhesion, Invasion, and Transcytosis of , with Host Cells a secreted toxin, adhesion to and/or invasion of host cells is necessary for colonization of the nasopharynx and subsequently to cause opportunistic disease in its human host. Knowledge of how pneumococci interact with host cells thereby helps to explain its biology and may identify potential targe