Titlebook: Stem Cells and Tissue Repair; Methods and Protocol Chrissa Kioussi Book 2020Latest edition Springer Science+Business Media, LLC, part of Sp

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Long-Term Ex Vivo Expansion of Murine Spermatogonial Stem Cells in a Simple Serum-Free Medium,ozoa in males. SSCs hold a unique position among tissue-specific stem cells in adults because of their ability to transmit the genetic information to subsequent generations. Ex vivo expansion of SSCs in conjunction with their transplantation is highly invaluable to study SSCs and develop new reprodu

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Generating Kidney Organoids from Human Pluripotent Stem Cells Using Defined Conditions,herapies for a wide range of intractable disorders. With the availability of human pluripotent stem cells (hPSCs) and greatly improved protocols for their directed differentiation into specific cell types, including kidney, this prospect could soon become a reality. We have previously described the

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Induction of Osteoblasts by Direct Reprogramming of Mouse Fibroblasts,tem cells (ESCs) and induced pluripotent stem cells (iPSCs). However, differentiation of osteoblasts from PSCs is time-consuming and low yield. In contrast, we identified four osteogenic transcription factors, Runx2, Osx, Dlx5, and ATF4, that rapidly and efficiently reprogram mouse fibroblasts deriv

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Preparation of Bioscaffolds Delivering Stem Cells for Neural Regeneration,binatorial implantation of neural conduits and iPSCs may significantly enhance neural regeneration. The repair of nerves and spinal cords using biodegradable multichannel collagen conduits has been reported in our previous studies. In this review, we describe a method to fabricate a collagen neural conduit containing iPSC-derived neural cells.

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Healing of Full-Thickness Murine Skin Wounds Containing Nanofibers Using Splints for Efficient Reepng contraction during healing. The rising popularity of nanofiber-based treatments for wound healing through sustained release of factors/molecules promoting wound closure can be potentially implemented in association with this model to determine its efficacy in wound management in a more humanized way.

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