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Titlebook: Stem Cells and Aging; Methods and Protocol Kursad Turksen Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Na

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书目名称Stem Cells and Aging
副标题Methods and Protocol
编辑Kursad Turksen
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Stem Cells and Aging; Methods and Protocol Kursad Turksen Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Na
描述This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy to follow fashion so as to be valuable for not only experts but novices in the ever-developing stem cell field. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and up-to-date, .Stem Cells and Aging: Methods and Protocols, Second Edition. serves as an ideal guide for all researchers working in this very active area of study..
出版日期Book 2019Latest edition
关键词Skeletal muscle cells; Progenitor Cells; Mesenchymal Stem Cells; Chromatin Immunoprecipitation; Epigenet
版次2
doihttps://doi.org/10.1007/978-1-4939-9713-8
isbn_softcover978-1-4939-9715-2
isbn_ebook978-1-4939-9713-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

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Methods and Strategies for Procurement, Isolation, Characterization, and Assessment of Senescence ogenerative medicine. These cells are also an efficient model to study biological pathways of stem cell action, tissue injury and disease. Like any other primary somatic cells in culture, industrial-scale expansion of mesenchymal stromal cells (MSC) leads to the replicative exhaustion/senescence as d
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Quantifying Senescence-Associated Phenotypes in Primary Multipotent Mesenchymal Stromal Cell Culturccumulate with advancing age; however, excessive/aberrant accumulation of senescent cells can disrupt normal tissue function. Multipotent mesenchymal stromal cells (MSCs), which are actively evaluated as cell-based therapy, can undergo replicative senescence or stress-induced premature senescence. T
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Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells,ities, MSC populations must be rapidly expanded to satisfy clinical needs. However, senescence attributed to extensive in vitro expansion compromises the regenerative and therapeutic potential of MSCs. In this chapter, we describe a step-by-step protocol that aims to induce adipogenic and osteogenic
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Human Skeletal Muscle-Derived Mesenchymal Stem/Stromal Cell Isolation and Growth Kinetics Analysis,g source of diverse subpopulations of MSCs, such as paired box 7 (Pax-7)-positive satellite cells, fibro-/adipogenic progenitors, PW1-positive interstitial cells and others. The specific properties of some of these muscle-derived cells have encouraged the development of cell therapies for muscle reg
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