Titlebook: Stem Cell Mobilization; Methods and Protocol Gerd Klein,Patrick Wuchter Book 2019 Springer Science+Business Media, LLC, part of Springer Na

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Colony Formation: An Assay of Hematopoietic Progenitor Cells, the colonies provide preliminary information about the ability of progenitors to differentiate and proliferate. To allow colonies to grow to a size which facilitates accurate counting and identification, about 14 days of culture is sufficient. In certain situations also shorter periods may be used.

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1064-3745 expertsThis book provides detailed state-of-the-art protocols for analyzing individual aspects of stem cell mobilization at the molecular and cellular level with contributions encompassing the most recent technological developments. Specifically, the volume covers cell adhesion and migration assays

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Evolution of Peripheral Blood Stem Cell Transplantation, the bone marrow are as follows: harvesting blood stem cells is less painful for the donor, utilizes much less resources such as operating theater time and general anesthesia, and, above all, is associated with .. The latter has ultimately improved patient safety as a consequence of significantly sh

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In Vitro Dynamic Phenotyping for Testing Novel Mobilizing Agents,ifferent from the microscopy-based high-content screening relying on multiple staining, machine learning, and molecular-level perturbation, the proposed method sheds light on the “dynamics” of HSPC in the presence of extrinsic factors, including SDF1α and mobilization agents. A well-defined model of

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Colony Formation: An Assay of Hematopoietic Progenitor Cells,ility to form colonies in a semisolid medium. The resulting colonies are consisting of more differentiated cells, and the number and the morphology of the colonies provide preliminary information about the ability of progenitors to differentiate and proliferate. To allow colonies to grow to a size w

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Transmigration Assays for the Determination of Molecular Interactions Between Hematopoietic Stem Cehematopoiesis in the transplanted host, to proliferate, to differentiate, and to successfully repopulate the hematopoietic system of a transplanted host. Stimulating agents shown to induce the transmigration of HSPC were often later identified to play significant roles in mobilization of HSPC or the