Titlebook: Stem Cell Mobilization; Methods and Protocol Gerd Klein,Patrick Wuchter Book 2019 Springer Science+Business Media, LLC, part of Springer Na

DEAWL

放大

A Microcavity Array-Based 3D Model System of the Hematopoietic Stem Cell Niche,still far from being fully understood. One reason is that hematopoiesis is a multi-step maturation process leading to HSPC heterogeneity. Subpopulations of HSPCs can be identified by clonogenic assays or in serial transplantation experiments in mice following sublethal irradiation, but it is very co

Prosaic

共栖

高贵领导

Assessing Cellular Hypoxic Status In Situ Within the Bone Marrow Microenvironment,hypothesized to be very limited. The hypoxic nature of HSC niches is proposed to play a fundamental role in the preservation of fundamental stem cell properties through the induction of a distinct glycolytic metabolic profile in HSCs. Thus, the capacity to determine oxygen levels or cellular oxygena

蚀刻术

bile648

Assessment of Proteolytic Activities in the Bone Marrow Microenvironment, marrow that has a strong influence on adhesive and chemotactic interactions of HSC with their niches. The increase of proteases during mobilization goes along with a decrease of endogenous protease inhibitors. Prominent members of the proteases involved in HSC mobilization belong to the families of

Mechanics

细丝

纤细

Assessment of Young and Aged Hematopoietic Stem Cell Activity by Competitive Serial Transplantationd incidence of blood-related diseases. Age-related phenotypes have been widely studied by bone marrow transplantation experiments, where reconstitution of the transplanted cells is a direct measure of HSC activity. In this protocol we describe a competitive bone marrow transplantation assay to funct