Titlebook: Spiritual Needs in Research and Practice; The Spiritual Needs Arndt Büssing Book 2021 The Editor(s) (if applicable) and The Author(s), und

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e positions that are crucial for the aptamer affinity. In this chapter, we provide a protocol to simultaneously study and improve an aptamer using Doped-SELEX and HTS analysis, including the design of the doped library, the selection, HTS, and analysis. This protocol could be useful to improve the a

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Arndt Büssing,Simon Peng-Kellerre binding events and to determine kinetic constants. In this chapter, a detailed protocol for the determination of kinetic constants for protein-aptamer interaction is provided. An 82-nt long ssDNA aptamer which are targeted against human urokinase is used as a model system for determination of bin

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Olga Riklikienėfluorogenic aptamers that exploits distance- and orientation-dependent energy transfer efficiencies between site-specifically incorporated fluorescent nucleoside analogs and non-covalently bound ligands, exemplified by 4-cyanoindol riboside (4CI) and the DMHBI.-binding RNA aptamer Chili. This method

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instructive

kinds of nuclear reactions, including measurements of reactions with neutrons and γ-rays not yet fully considered in previous compilations. The nuclei considered also comprise nuclei far from the stability line.. .The properties of excited nuclear states are of importance for scientific and practica

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Arndt Büssingtional scientists.Also available online on http://materials..Volume I/25E is the last one in a series of volumes on the properties of the excited states of all nuclei. The data presented are collected from all kinds of nuclear reactions, including measurements of reactions with neutrons and γ-rays n

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tional scientists.Also available online on http://materials..Volume I/25E is the last one in a series of volumes on the properties of the excited states of all nuclei. The data presented are collected from all kinds of nuclear reactions, including measurements of reactions with neutrons and γ-rays n

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