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Titlebook: Spectroscopic Methods of Analysis; Methods and Protocol Wlodek M. Bujalowski Book 2012 Springer Science+Business Media New York 2012 RNA fo

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发表于 2025-3-21 19:18:35 | 显示全部楼层 |阅读模式
书目名称Spectroscopic Methods of Analysis
副标题Methods and Protocol
编辑Wlodek M. Bujalowski
视频video
概述Outlines important and timely biological problems, as predominantly tackled by optical spectroscopic methods.Provides step-by-step detail essential for reproducible results.Contains key notes and impl
丛书名称Methods in Molecular Biology
图书封面Titlebook: Spectroscopic Methods of Analysis; Methods and Protocol Wlodek M. Bujalowski Book 2012 Springer Science+Business Media New York 2012 RNA fo
描述.Quantitative elucidation of structural, energetic and dynamic aspects of macromolecular interactions is indispensable for understanding the functional activities of biomolecules and their interactions. The optical spectroscopic methods are not confined to small molecules or macromolecules but permit the studies of even the largest biological systems in their full splendor, including the living cell. In, .Spectroscopic Methods of Analysis: Methods and Protocols., expert researchers in the field detail many of the methods which are now commonly used to study properties of individual macromolecules, their complexes, organelles, and cells, using optical spectroscopic techniques. These include methods and approaches for experimental and theoretical analyses of fluorescence properties of the examined systems, single molecule approaches, electronic absorption, and electro-optical analyses of macromolecular interactions, structures, and dynamics. Written in the highly successful .Methods in Molecular Biology.(tm) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubl
出版日期Book 2012
关键词RNA folding; Single molecule; biological systems; birefringence; electric dichroism; electro-optical anal
版次1
doihttps://doi.org/10.1007/978-1-61779-806-1
isbn_softcover978-1-4939-6141-2
isbn_ebook978-1-61779-806-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2012
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978-1-4939-6141-2Springer Science+Business Media New York 2012
发表于 2025-3-22 01:41:06 | 显示全部楼层
Spectroscopic Methods of Analysis978-1-61779-806-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Methods in Molecular Biologyhttp://image.papertrans.cn/s/image/873930.jpg
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https://doi.org/10.1007/978-1-61779-806-1RNA folding; Single molecule; biological systems; birefringence; electric dichroism; electro-optical anal
发表于 2025-3-22 18:50:21 | 显示全部楼层
Fluorescence Methods to Study DNA Translocation and Unwinding Kinetics by Nucleic Acid Motors,e intensity or efficiency of fluorescence resonance energy transfer (FRET). We discuss how the pre-steady state kinetic data collected in these ensemble experiments can be analyzed by simultaneous global nonlinear least squares (NLLS) analysis using simple sequential “.” mechanisms to obtain estimat
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Fluorescence Intensity, Anisotropy, and Transient Dynamic Quenching Stopped-Flow Kinetics,ing to the . replicative helicase, the DnaB protein..Fluorescence intensity is one of the most often used spectroscopic signals to monitor the progress of biochemical reactions. Its properties also give an opportunity to address various structural aspects of the intermediates unavailable by any othe
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,Using Structure–Function Constraints in FRET Studies of Large Macromolecular Complexes,the parameter, .., which depends on the mutual orientation of the donor and the acceptor. Known procedures, based on limiting anisotropy measurements, to estimate .. are of limited use to deducing the functional conclusions about the studied systems. On the other hand, using multiple donor–acceptor
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Illuminating Allostery in Metal Sensing Transcriptional Regulators,pically derived methods for measuring metal binding affinity, .., as well as Δ.. independent of .., presented in the context of a simple coupled equilibrium scheme. Studies carried out in this way provide quantitative insights into the degree to which a particular metal ion is capable of driving all
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