Titlebook: Solanum tuberosum; Methods and Protocol David Dobnik,Kristina Gruden,Anna Coll Book 2021 Springer Science+Business Media, LLC, part of Spri

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RNA Sequencing Analyses for Deciphering Potato Molecular Responsesp yields. RNA sequencing (RNA-Seq) provides an insight into responses of all of the organism genes to the environmental and developmental cues and thus provides insights into underlying modes of action. In this chapter, we guide a researcher through some of the most important steps in the analysis o

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Yeast Two-Hybrid Screening for Identification of Protein-Protein Interactions in ,plit transcription factor into close proximity, thereby reconstituting its ability to activate reporter genes. There are many variations on this technique. Here we provide an adapted protocol based on the Invitrogen ProQuest™ Two-Hybrid system, which has been used successfully to screen over 60 . pa

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Potato as a Model for Field Trials with Modified Gene Functions in Research and Translational Experine functions. Field trial following laboratory experiments is an important step in order to evaluate new functions since many phenotypes, and combinations thereof, are difficult to detect in controlled environments and molecular analyses are nowadays possible to do in the field. Here we describe a s

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MapMan Visualization of RNA-Seq Data Using Mercator4 Functional Annotationsto biological processes. This impressive array of data can prove challenging to interpret. In this chapter, we describe a solution to this problem, consisting of the MapMan transcript visualization application and the associated MapMan4 ontology and Mercator4 online annotation process.

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Co-expression for Genotype-Phenotype Function Annotation in Potato Research bait genes and transcriptome datasets in the Cytoscape plug-in, the analysis is run and the resulting network is displayed immediately. Using the web tool, the user can download the Cytoscape files and import them manually into the program..The easiest way to calculate correlations for your data is