Titlebook: Skin Tissue Engineering; Methods and Protocol Sophie Böttcher-Haberzeth,Thomas Biedermann Book 2019 Springer Science+Business Media, LLC, p

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Three-Dimensional Epidermal Model from Human Hair Follicle-Derived Keratinocytes models, not only in toxicology, but also in skin biology. Although there are currently several reconstructed human epidermis (RHE) models commercially available, the donors of the keratinocytes are not identified in these models. A tailor-made system is needed to investigate the individual differen

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Fabrication of a Co-Culture System with Human Sweat Gland-Derived Cells and Peripheral Nerve Cellserature control, and wound healing. Here we describe a protocol for the setup of an indirect co-culture system of peripheral nerve cells and sweat gland-derived stem cells, which can be used to quantify neurite outgrowth.

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Engineering a Multilayered Skin Substitute with Keratinocytes, Fibroblasts, Adipose-Derived Stem Cel clinical application lies in dermal and epidermal substitutes, the development of a subcutaneous replacement, the hypodermis, is often neglected. This chapter describes the use of fibrin sealant as a hydrogel scaffold to generate a three-dimensional skin substitute. For the hypodermal layer adipose

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Fabrication of Chimeric Hair Follicles for Skin Tissue Engineeringng the functionality of current skin substitute models requires incorporation of skin appendages, including hair follicles, sebaceous glands, and sweat glands. In this chapter, methods for generating skin substitutes incorporating chimeric hair follicles are described. Isolation of human keratinocyt

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Isolation and Culture of Epidermolysis Bullosa Cells and Organotypic Skin Modelsadhesion defects of these cells. We routinely employ a well-established protocol for in vitro culture of these cells from small skin samples remaining after diagnostic procedures are performed. Keratinocytes and fibroblast can be used for downstream expression and functional studies or for construct

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Effects of the Extracellular Matrix on the Proteome of Primary Skin Fibroblasts biallelic mutations of the gene ., encoding for collagen VII, the main component of anchoring fibrils, lead to a loss of collagen VII in the extracellular matrix (ECM). Loss of collagen VII in skin is linked to a destabilization of the dermal-epidermal junction zone, blister formation, chronic woun

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Standard Preparation Protocol of Human Skin Samples for Transmission Electron Microscopytive sample preparation protocol for transmission electron microscopy (TEM). Critical steps to obtain good ultrastructure are mentioned as well as notes to improve the sample collection. New ways to automatize the imaging acquisition are discussed.

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