Titlebook: Skin Tissue Engineering; Methods and Protocol Sophie Böttcher-Haberzeth,Thomas Biedermann Book 2019 Springer Science+Business Media, LLC, p

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Isolation and Culture of Human Dermal Fibroblastsrimary fibroblast is gaining in importance. In addition, fibroblasts established from skin biopsies provide a powerful tool for investigating normal skin physiology or specific disease states. In this chapter, detailed procedures for establishing and maintaining primary cultures of adult human dermal fibroblasts are described.

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Isolation and Culture of Human Dermal Microvascular Endothelial Cellstain and isolate. An alternative is the use of human dermal microvascular endothelial cells, more representative of adult skin angiogenesis and vascularization processes. This chapter presents a detailed methodology to isolate and culture microvascular endothelial cells from skin biopsies based on enzymatic digestion and mechanical extraction.

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Isolation and Culture of Human Keratinocyteslso used to investigate various cellular and molecular mechanisms involved in different skin pathologies. To preserve stem cells during epithelial cell culture, reliable methods and conditions are of the utmost importance. Properly cultured keratinocytes will exhibit a consistent cuboid morphology a

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Isolation and Culture of Hair Follicle Dermal Sheath Mesenchymal Stromal Cellsrmal sheath (DS). We present protocols for the isolation and culture of human DS-MSCs starting with the use of a dissecting microscope to separate out dermal sheaths from hair follicles for trypsin digestion. We also present the protocols for the adipogenic, osteogenic, and chondrogenic differentiat

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Isolation and Culture of Human Dermal Fibroblastst and in interfollicular skin. Moreover, they play an essential role during cutaneous wound healing and in bioengineering of skin. Hence, culture of primary fibroblast is gaining in importance. In addition, fibroblasts established from skin biopsies provide a powerful tool for investigating normal s

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Isolation and Culture of Human Dermal Microvascular Endothelial Cellstissues. Investigations often use human umbilical vein endothelial cells due to their extensive characterization, but also because they are easy to obtain and isolate. An alternative is the use of human dermal microvascular endothelial cells, more representative of adult skin angiogenesis and vascul