Titlebook: Skeletal Muscle Development; James G. Ryall Book 2017 Springer Science+Business Media LLC 2017 Cell proliferation.Myogenic differentiation

谄媚于人

Whole Genome Chromatin IP-Sequencing (ChIP-Seq) in Skeletal Muscle Cells fragmented chromatin, followed by high-throughput sequencing to identify the protein bound regions genome-wide. Here, in this protocol, we will illustrate how the entire ChIP-seq is performed using global H3K27ac profiling in myoblast cells as an example.

crescendo

isotope

冥界三河

Membrane Repair Assay for Human Skeletal Muscle Cellsane disruption and repair are assayed by monitoring the incorporation into myotubes of the membrane probe FM1-43. This methodology has recently enabled us to show that Annexin-A5 is required for membrane repair in human skeletal muscle cells (Carmeille et al., Biochim Biophys Acta 1863:2267–2279, 2016).

Neolithic

Measuring Mitochondrial Substrate Utilization in Skeletal Muscle Stem Cellsto measure mitochondrial respiration and lactate production. In this chapter, we describe the use of key metabolic inhibitors that allow for the investigation of mitochondrial substrate utilization in primary MuSCs.