Titlebook: Skeletal Development and Repair; Methods and Protocol Matthew J. Hilton Book 2021Latest edition Springer Science+Business Media, LLC, part

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Murine Limb Bud Organ Cultures for Studying Musculoskeletal Developmentude a number of vital embryonic signaling pathways, including the fibroblast growth factor, WNT, transforming growth factor, and hedgehog. Collectively these signals converge on multiple progenitor populations to drive the formation of a variety of tissues that make up the limb musculoskeletal syste

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Renal Capsule Transplantation to Assay Angiogenesis in Skeletal Development and Repairnt and biological analyses. This chapter describes the surgical procedure for the transplantation of embryonic skeletal elements in the renal capsule of adult mice and points out the manipulations that can be applied for assaying the role of angiogenesis during bone development and repair.

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Phenotyping Intact Mouse Bones Using Bone CLARITYaborious and destructive, as the native 3D environment of the bone is not maintained. Presented here is a bone-clearing methodology, termed Bone CLARITY, which combines published techniques for clearing soft tissues, including delipidation for the removal of light-scattering membranes, hydrogel-embe

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