Titlebook: Single-Stranded DNA Binding Proteins; Methods and Protocol James L. Keck Book 2012 Springer Science+Business Media, LLC 2012 DNA-binding pr

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Ammonium Sulfate Co-precipitation of SSB and Interacting Proteins, are mediated by the essential and conserved amphipathic C-terminus (SSB-Ct). SSB plays a critical role in localizing and stimulating the activity of a wide variety of DNA-processing proteins. The interaction partners have been identified and studied using a variety of methods, one of which, ammoniu

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Analyzing Interactions Between SSB and Proteins by the Use of Fluorescence Anisotropy,ing fluorescently labeled SSB C-terminal tail (SSB-Ct) peptide results in formation of the protein complex with molecular weights greatly higher than SSB peptide alone. Thus the tumbling of the complexes in solution is slower, and the fluorescence anisotropy (FA) signal would be increased, indicatin

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Use of Native Gels to Measure Protein Binding to SSB, the interaction of . (.) SSB with its cognate RecO protein. The interaction is detected as decay of the band corresponding to SSB by addition of RecO. We also demonstrate analysis of the RecO–RecR interaction as another example of this method.

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Detection of Posttranslational Modifications of Replication Protein A,ifications including serine- and threonine-phosphorylation, poly-ADP ribosylation, and SUMOylation. These modifications are believed to regulate RPA activity through modulating interactions with DNA and partner proteins. This article describes two methods used to detect posttranslational modified RP

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