书目名称 | Single-Stranded DNA Binding Proteins | 副标题 | Methods and Protocol | 编辑 | James L. Keck | 视频video | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .The genomes of cellular organisms are organized as double-stranded DNA, a structure that must be unwound to provide DNA replication, recombination, and repair machinery access to genomic information. However, DNA unwinding comes with inherent risks to genome stability. To help mediate these risks, bacterial, archael, and eukaryotic cells have evolved protective ssDNA-binding proteins (SSBs) that bind ssDNA with high affinity and specificity. SSBs also aid genome metabolic processes through direct interactions with key proteins in genome maintenance enzymes. .Single-Stranded DNA Binding Proteins: Methods and Protocols. assembles methods developed for examining the fundamental properties of SSBs and for exploiting the biochemical functions of SSBs for their use as in vitro and in vivo reagents. Clearly and concisely organized, the volume opens with an introduction to the structures and functions of SSBs, followed protocols for studying SSB/DNA complexes, methods for studying SSB/heterologous protein complexes, protocols for interrogating post-translational modifications of SSBs, and concludes with uses of fluorescently-labeled SSBs for in vitro and in vivo studies of genome main | 出版日期 | Book 2012 | 关键词 | DNA-binding proteins; SSB proteins; genome maintenance enzymes; heterologous protein complexes; ssDNA; Pr | 版次 | 1 | doi | https://doi.org/10.1007/978-1-62703-032-8 | isbn_softcover | 978-1-4939-6251-8 | isbn_ebook | 978-1-62703-032-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC 2012 |
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