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Titlebook: Single-Channel Recording; Bert Sakmann,Erwin Neher Book 1983 Plenum Press, New York 1983 activation.art.cell.cell culture.enzyme.influence

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书目名称Single-Channel Recording
编辑Bert Sakmann,Erwin Neher
视频video
图书封面Titlebook: Single-Channel Recording;  Bert Sakmann,Erwin Neher Book 1983 Plenum Press, New York 1983 activation.art.cell.cell culture.enzyme.influence
描述Single-channel recording has become a widely used tool for the study of ion permeation mechanisms in biological membranes. Whereas the technique might have been considered an "art" after its introduction in 1976, it developed into a relatively simple method after it became possible to obtain high-resistance (several gigaohm) membrane-pipette seals. In the summer of 1982, a course on the technique was held at the Ettore Majorana Center for Scientific Culture in Erice, Sicily. It brought together people from most of the laboratories involved in patch clamping at that time. During the course, it became apparent that the technique had reached a state of maturity. Repeatedly, the opinion was expressed that a detailed description of all the aspects of the technique including representative examples of results should be available. We therefore asked the course instructors, as well as several other colleagues, to provide chapters on selected topics in order to produce this volume. The different variants of patch clamping were described quite extensively in an article by Hamill, Marty, Neher, Sakmann, and Sigworth (Pflugers Archiv 391:85) in 1981. Rather than repeating this survey in an int
出版日期Book 1983
关键词activation; art; cell; cell culture; enzyme; influence; liposomes; membrane; muscle; neurons; resistance; time;
版次1
doihttps://doi.org/10.1007/978-1-4615-7858-1
isbn_softcover978-1-4615-7860-4
isbn_ebook978-1-4615-7858-1
copyrightPlenum Press, New York 1983
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Enzymatic Dispersion of Heart and Other Tissuesfor the patch clamp technique. They have a cell surface stripped of connective tissue, a prerequisite for the formation of a gigaseal between the current-recording pipette and the cell membrane (Neher, 1982).
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Geometric Parameters of Pipettes and Membrane Patchesof the optical microscope. Only rarely is it possible to “see” the membrane patch during the experiment, and even then quantitative evaluation of its size is impossible or difficult. The purpose of this chapter is to provide some hints toward estimation of the tip geometry of patch pipettes and of p
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Science and Technology of Patch-Recording Electrodes tightly to the tip of a glass microelectrode. Because the seal is electrically tight, electrical noise is reduced by several orders of magnitude. Because the glass-membrane bond is mechanically strong, the patch of membrane spanning the tip may be isolated from the cell, or the patch may be rupture
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Enzymatic Dispersion of Heart and Other Tissueserry and Friend, 1969; cardiac myocytes: Vahouny ., 1970; smooth muscle cells: Bagby ., 1971). Most of the procedures have been designed to meet the needs of biochemical investigations. For electrophysiologists, enzymatic dissociation is of interest if the cells are not easily accessible otherwise o
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