Titlebook: Single-Channel Recording; Bert Sakmann,Erwin Neher Book 1983 Plenum Press, New York 1983 activation.art.cell.cell culture.enzyme.influence

公式

A Primer in Cell Culture for Patchologistsehavior of excitable membranes has been gained from studies of a few nonmammalian preparations that are highly suitable for conventional voltage clamp experiments. Many of the cultured systems have simply been ignored, whereas others have been unsuitable for rigorous studies using conventional electrophysiological techniques.

有机体

The Principles of the Stochastic Interpretation of Ion-Channel Mechanismsle reaction step, a problem that is considered in Section 7. Our primary aim is to gain insight about the nature of the reaction mechanism from experimental observations. In this process, we may also obtain estimates of numerical values for the rate constants in the mechanism.

门窗的侧柱

amnesia

空洞

PHAG

冬眠

Tight-Seal Whole-Cell Recordings established between the pipette and the cell interior, which is well insulated from the bath. It is our experience that this way of penetrating a cell inflicts much less damage on the cell than the standard microelectrode impalement.

precede

The Loose Patch Clamptage-clamp techniques, with loss of the positional information. Also, the “gigaseals” needed for single-channel recording require extensive proteolytic enzymatic treatment on many cells in order to clean the membrane surface.

防御

Conformational Transitions of Ionic Channelspendent of time and not influenced by the movement of the ion. This description, which corresponds to an essentially static picture of protein structure, represents a useful approximation in certain cases. Recent findings on the dynamics of proteins, however, suggest a more general concept of barrier structure.

employor