Titlebook: Single Cell Transcriptomics; Methods and Protocol Raffaele A. Calogero,Vladimir Benes Book 2023 The Editor(s) (if applicable) and The Autho

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Bringing Cell Subpopulation Discovery on a Cloud-HPC Using rCASC and StreamFlow,ing libraries in which the transcripts are tagged to track their cell of origin. Modern scRNA-seq platforms are capable of analyzing up to many thousands of cells in each run. Then, combined with massive high-throughput sequencing producing billions of reads, scRNA-seq allows the assessment of funda

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Practical Considerations for Complex Tissue Dissociation for Single-Cell Transcriptomics,ell types, and mapping of development as well as disease states. Successful application of this technology critically relies on the dissociation of solid organs and tissues into high-quality single-cell (or nuclei) suspensions..In this chapter, we examine several key aspects of the tissue handling w

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Functional-Feature-Based Data Reduction Using Sparsely Connected Autoencoders,lying biology since scRNA-seq data are characterized by high noise. In this chapter, we describe a functional feature-driven data reduction approach, which could provide a better link among cell clusters and their underlying cell biology.

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1064-3745 ation advice from the expertsThis volume provides up-to-date methods on single cell wet and bioinformatics protocols based on the researcher experiment requirements. Chapters detail basic analytical procedures, single-cell data QC, dimensionality reduction, clustering, cluster-specific features sele

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Integration of scATAC-Seq with scRNA-Seq Data,bility at the single-cell level. However, the integration of multi-omics data still remains one of the most difficult tasks in bioinformatics. In this chapter, we focus on the combination of scRNA-Seq and scATACSeq data to perform an integrative analysis of the single-cell transcriptome and chromatin accessibility of human fetal progenitors.

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Menschenrechte. Zwischen normativer Geltung und politischer Verbindlichkeith diskutiert wird. Selbst wo Menschenrechte als Normen begriffen werden wie im Völkerrecht und in großen Teilen der Philosophie, kann »Recht« unterschiedliches meinen: gerichtsfeste individuelle Rechtansprüche oder aber moralische Ansprüche, die einer öffentlichen Begründung stand halten müssen.