Titlebook: Single Cell Methods; Sequencing and Prote Valentina Proserpio Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019

Blood-Clot

Hectic

心胸开阔

Full-Length Single-Cell RNA Sequencing with Smart-seq2 characterization of rare cell types as well as the detailed analysis of the stochastic nature of gene expression. A large number of methods have been developed, varying in their throughput, sensitivity, and scalability. A major distinction is whether they profile only 5′- or 3′-terminal part of the

Hemoptysis

拒绝

Single-Cell RNA-Seq by Multiple Annealing and Tailing-Based Quantitative Single-Cell RNA-Seq (MATQ-S heterogeneity in the transcriptome, high accuracy and sensitivity are still desired for single-cell RNA-seq. We describe here multiple annealing and dC-tailing-based quantitative single-cell RNA-seq (MATQ-seq) with ~90% capture efficiency. In addition, MATQ-seq is a total RNA assay allowing for det

expire

Single-Cell RNA Sequencing with Drop-Seqcapture microparticles and cells are coconfined through a microfluidic device within the droplets where they undergo cell lysis and RNA hybridiztion. After breaking the droplets and pooling the hybridized particles, reverse transcription, PCR, and sequencing in single reactions allow to generate dat

受辱

Chromium 10× Single-Cell 3′ mRNA Sequencing of Tumor-Infiltrating Lymphocytesin parallel. Chromium10× by 10× Genomics is an emulsion-based device that enables to compartmentalize single cells along with sets of uniquely barcoded primers and reverse transcription reagents into nanoscale droplets that are used as reaction chambers to generate barcoded full-length cDNA from sin

呼吸

Seq-Well: A Sample-Efficient, Portable Picowell Platform for Massively Parallel Single-Cell RNA Sequlinical samples. In Seq-Well, uniquely barcoded mRNA capture beads and cells are co-confined in picowells that are sealed using a semipermeable membrane, enabling efficient cell lysis and mRNA capture. The beads are subsequently removed and processed in parallel for sequencing, with each transcript’

马赛克

Book 2019ailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respectiv

Cabinet

Book 2019e topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Single Cell Methods: Sequencing and Proteomics. aims to make each experiment easily reproducible in every lab..   .