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Titlebook: Single Cell Methods; Sequencing and Prote Valentina Proserpio Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019

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发表于 2025-3-21 18:03:13 | 显示全部楼层 |阅读模式
书目名称Single Cell Methods
副标题Sequencing and Prote
编辑Valentina Proserpio
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Single Cell Methods; Sequencing and Prote Valentina Proserpio Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019
描述.This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Single Cell Methods: Sequencing and Proteomics. aims to make each experiment easily reproducible in every lab..   .
出版日期Book 2019
关键词PCR; Drop-seq; sc-ChIP-seq; Crispr-Screening; Fisseq
版次1
doihttps://doi.org/10.1007/978-1-4939-9240-9
isbn_softcover978-1-4939-9242-3
isbn_ebook978-1-4939-9240-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
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1064-3745 ation advice from the experts.This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, si
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Tissue Handling and Dissociation for Single-Cell RNA-Seqmmune cells from lymphoid and nonlymphoid tissues to be then used as input to single-cell methods. We highlight the main factors that can influence the final quality of single-cell data, namely the stress signatures that can bias its interpretation.
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Single-Cell RNA Sequencing with Drop-SeqAfter breaking the droplets and pooling the hybridized particles, reverse transcription, PCR, and sequencing in single reactions allow to generate data from thousands of single-cell transcriptomes while maintaining information on the cellular origin of each transcript.
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Seq-Well: A Sample-Efficient, Portable Picowell Platform for Massively Parallel Single-Cell RNA Sequne, enabling efficient cell lysis and mRNA capture. The beads are subsequently removed and processed in parallel for sequencing, with each transcript’s cell of origin determined via the unique barcodes. Due to its simplicity and portability, Seq-Well can be performed almost anywhere.
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Chromium 10× Single-Cell 3′ mRNA Sequencing of Tumor-Infiltrating Lymphocytese main steps are cell preparation, cDNA synthesis, library construction, and sequencing..This protocol refers specifically to the CG00052_SingleCell3_ReagentKitv2UserGuide_RevD downloadable from 10× Genomics website (.) and does not substitute it. Always refer to this guide, paying attention to updates and revisions.
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