Titlebook: Single Cell Metabolism; Methods and Protocol Bindesh Shrestha Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020

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Identification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry,cell-to-cell heterogeneity. However, identifying biomolecules in single cells presents significant challenges due to the low picoliter volume samples and the structural diversity of metabolites. Capillary microsampling electrospray ionization (ESI) MS with ion mobility separation (IMS) enables the a

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Analysis of Lipids in Single Cells and Organelles Using Nanomanipulation-Coupled Mass Spectrometry,biomarkers. Some major challenges of individual cell analysis include requirement and expenditure of a large sample of cells as well as extensive extraction and separation techniques. Here, we describe methods to perform individual cell and organelle extractions of both tissues and cells in vitro us

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Development of Pico-ESI-MS for Single-Cell Metabolomics Analysis,eved by pulsed direct current electrospray ionization source (Pulsed-DC-ESI). This source could collect MS signals for a few minutes from a cell, enabling us to obtain large-scale MS. data of metabolite IDs in single-cell analysis. Further identification of the single-cell metabolome such as the dat

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Single-Probe Mass Spectrometry Analysis of Metabolites in Single Cells, used for sensitive detection and accurate identification of species of interest. In traditional studies, MS is utilized to measure analytes in prepared solutions or gas-phase samples. Benefited from recent development of sampling and ionization approaches, MS has been extensively applied to the ana

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Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging,that can be visualized simultaneously. Here, we highlight a protocol to visualize lipids from single cardiac cells using high-resolution atmospheric-pressure matrix-assisted laser desorption/ionization coupled to an orbital trapping mass analyzer.

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Sample Preparation and Analysis of Single Cells Using High Performance MALDI FTICR Mass Spectrometr to microscopy. This unique combination of capabilities is ideally suited for the analysis of metabolites and lipids from single cells. This chapter describes a methodology for the sample preparation and analysis of single cells using high performance MALDI FTICR MS. Using this approach, we are able

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