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Titlebook: Single Cell Analysis; Methods and Protocol Miodrag Gužvić Book 2024 Springer Science+Business Media, LLC, part of Springer Nature 2024 qPCR

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发表于 2025-3-21 17:08:56 | 显示全部楼层 |阅读模式
书目名称Single Cell Analysis
副标题Methods and Protocol
编辑Miodrag Gužvić
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Single Cell Analysis; Methods and Protocol Miodrag Gužvić Book 2024 Springer Science+Business Media, LLC, part of Springer Nature 2024 qPCR
描述This volume explores the latest advancements and techniques used to study cell analysis, their capabilities, and the type of results that can be obtained. The chapters in this book cover topics such as FACS; fluorescence microscopy; organic spectroscopy such as MALDI; inorganic spectroscopy such as ICP-MS; and sequencing. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and practical, .Single Cell Analysis: Methods and Protocols. is a valuable tool for any researcher interested in learning more about this important and developing field. .
出版日期Book 2024
关键词qPCR; western blot; Transcriptome; Mass Cytometry; Atomic Force Microscopy; ICP-MS
版次1
doihttps://doi.org/10.1007/978-1-0716-3621-3
isbn_softcover978-1-0716-3623-7
isbn_ebook978-1-0716-3621-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2024
The information of publication is updating

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978-1-0716-3623-7Springer Science+Business Media, LLC, part of Springer Nature 2024
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Single Cell Isolation from Surgically Resected Tissue Via Mechanical Dissociation Using TissueGrindsolid tissue are neither robust nor reproducible. Here we describe primary cells isolation from surgically resected tumor tissue via enzyme-free mechanical dissociation using TissueGrinder, a novel semi-automated benchtop device. The isolated cells can be used for any downstream biochemical or cell-based analytic assay.
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Isolation of Viable Epithelial and Mesenchymal Circulating Tumor Cells from Breast Cancer Patients,entrifugation, multiplex immunofluorescent staining, and negative anti-CD45 selection. Cells obtained after the procedure are suitable for genomic or transcriptomic profiling, and they can also be isolated by micromanipulation for single-cell analysis.
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