Titlebook: Signal Transduction — Single Cell Techniques; Bert Duijn,Anneke Wiltink Book 1998 Springer-Verlag Berlin Heidelberg 1998 ATP.Calcium.Endop

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is lab manual. An introductionary section on cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological secti

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A Modified U-Tube Drug Application Systemell, the demands on the system become even more complicated. Most drug application systems are based on a U-tube drug application device. The essential idea of this U-shaped loop is depicted in Fig. 4.1.30

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Book 1998ual. An introductionary section on cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustr

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A Modified U-Tube Drug Application Systemrequires a system allowing local and rapid application of the desired drug. Moreover, when the effects of more than one drug are studied on the same cell, the demands on the system become even more complicated. Most drug application systems are based on a U-tube drug application device. The essentia

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Maintaining Cells Under the Microscope time and analyzed in detail. In addition, physiological studies on populations of cells fail to detect the kinetics of these processes in the individual cell and the intercellular differences which are no doubt present.

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Book 1998ates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH.