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Titlebook: Signal Transduction and Protein Phosphorylation; L. M. G. Heilmeyer Conference proceedings 1987 Springer Science+Business Media New York 1

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Fluorescent Probes one type of molecules or ions. The concentration of the fluorescently labeled molecules or ions can be determined without interference from other molecules. Concentrations in the nanomolar range or below can be accurately determined. Advanced techniques and instruments have been developed by which
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Binding of Fluorescent Analogs of Cyclic GMP to cGMP-Dependent Protein Kinaseas four partially cooperative binding sites for cGMP with K.-values in the order of 10 to 200 nM as has been shown by binding studies with .H-cGMP.. Two types of sites have been described, site 1 with high affinity and slow dissociation and site 2 with lower affinity and faster dissociation. The pri
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Regulation of Muscle Contractionractile system are located in different structures, the thin actin filaments and the thick myosin filaments. The substrate for contraction. MgATP, is a poor substrate for myosin alone but when it interacts with actin it is split at a high rate. Thus regulation involves controlling the interaction of
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Binding of Fluorescent Analogs of Cyclic GMP to cGMP-Dependent Protein Kinasewo types of sites have been described, site 1 with high affinity and slow dissociation and site 2 with lower affinity and faster dissociation. The primary structure of the enzyme has been reported and assigned to functional domains..
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