Titlebook: Signal Transduction Protocols; Robert C. Dickson,Michael D. Mendenhall Book 20042nd edition Humana Press 2004

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Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets,ter, optimized protocols for 2-DE using extracts from tissue culture are provided. Protocols for in-gel digestion of gel-resolved proteins, which allow protein identification by mass spectrometry are also discussed.

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Determining Protein Half-Lives,cific physiological conditions. Over the years, procedures to determine the half-life of proteins in cultured eukaryotic cells have been well-established. This chapter describes in detail the two most frequently used methods, pulse-chase analysis and cycloheximide blocking, to determine a protein’s half-life in yeast and cultured mammalian cells.

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Methods in Molecular Biologyhttp://image.papertrans.cn/s/image/867101.jpg

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Signal Transduction Protocols978-1-59259-816-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Robert C. Dickson,Michael D. MendenhallIncludes supplementary material:

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Elaine A. Elion,Yunmei Wangir answers, which have been analyzed using discourse analysis methods, to identify, classify, and connect the main themes presented by respondents. Although the topics raised in various questions were quite different, it was possible to classify the respondents by their general attitude regarding th