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Titlebook: Signal Transduction Immunohistochemistry; Methods and Protocol Alexander E. Kalyuzhny Book 2023Latest edition The Editor(s) (if applicable)

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https://doi.org/10.1007/978-1-0716-2811-9Antibodies; Multiplex IHC; Flow cytometry; Signal transduction targets; Drug discovery; Biotechnology
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Alexander E. KalyuzhnyIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
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Immunohistochemical Techniques for Phosphoproteins,chnique allows detecting the phosphorylated state of signaling proteins in formalin-fixed and paraffin-embedded tissue sections by using phosphospecific antibodies. This chapter describes in detail the immunohistocshemical protocols from which the study of phosphoproteins in tissue sections can be approached.
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978-1-0716-2813-3The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Signal Transduction Immunohistochemistry978-1-0716-2811-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Visualizing Activated Myofibroblasts Resulting from Differentiation of Mesenchymal Stem Cells,DAMB231 human breast cancer cells. In this section, we show using immunofluorescence that keratinocyte-conditioned medium (KCM) induces differentiation of MSCs to resemble dermal myofibroblast-like cells with punctate vinculin staining and F-actin filaments.
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